Pairings shown with regional seed. All games on Friday, November 15, at 7 p.m.
Higher seeded team is the designated home team and listed first.
Division I
Region 2
- 5. Hilliard Davidson (8-4) vs. 8. Huber Heights Wayne (7-5) at Miami Trace High School Tony’s Welding and Fabrication Stadium
- Centerville (9-3) vs. 14. Dublin Coffman (4-8) at Springfield High School Wildcat Stadium
Region 4
- Cincinnati Archbishop Moeller (11-1) vs. 4. Cincinnati Princeton (10-2) at Mason High School Tri-Health/Beacon Stadium
- West Chester Lakota West (11-1) vs. 6. Cincinnati St. Xavier (9-3) at Cincinnati Princeton High School Jake Sweeney Automotive Stadium
Division II
Region 8
- Cincinnati Anderson (12-0) vs. 5. Cincinnati Mount Healthy (9-2) at Lakota East High School
- Cincinnati La Salle (10-2) vs. 6. Harrison (10-2) at Hamilton Ross High School
Division III
Region 12
- London (12-0) vs. 8. St. Marys Memorial (10-2) at Piqua High School Alexander Stadium
- Wapakoneta (12-0) vs. 3. Bellbrook (11-1) at Troy High School Memorial Stadium
Division IV
Region 16
- Cincinnati Taft (10-2) vs. 9. Cincinnati Indian Hill (9-3) at Milford High School Eagle Stadium Powered by Cullen Electric
- Cleves Taylor (10-2) vs. 10. Kettering Archbishop Alter (7-5) at Fairfield High School Christy Rose Dennis Stadium
Division V
Region 20
- Jamestown Greeneview (12-0) vs. 5. Lewistown Indian Lake (10-2) at Fairborn High School Skyhawk Stadium
- West Liberty-Salem (11-1) vs. 3. Waynesville (9-3) at Dayton Welcome Stadium
Division VI
Region 24
- Coldwater (11-1) vs. 5. St. Bernard Elmwood Place (10-2) at Trotwood-Madison High School Miami Valley Hospital SportsPlex
- Cincinnati Country Day (11-1) vs. 3. Anna (9-3) at Monroe High School Hornet Stadium
Division VII
Region 28
- Maria Stein Marion Local (12-0) vs. 4. Ansonia (11-1) at Lima Spartan Stadium
- Minster (10-2) vs. 3. Cincinnati College Prep (9-2) at Sidney High School Memorial Stadium